Recombinant Biotinylated Human SOST/Sclerostin Protein, His-Avi TagProduct Properties Synonyms VBCHsclerosteosis; SOST; sclerostin; DAND6 Source Biotinylated Human SOST Sclerostin Protein is expressed from HEK293 with His and Avi tag at the N Terminus. It contains Gln24 Tyr213.[Accession Q9BQB4 1] Molecular Weight The protein has a predicted MW of 24. 2 kDa. Due to glycosylation,the protein migrates to 28 40 kDa based on SDS PAGE result. Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by

His Tag with the EC50 of 31

and necrotic cells

the less likely it is to appear in the gene fragment

Annealing/Extension（Fluorescence collection）

the protein migrates to 45-50 kDa and 80-90 kDa based on Tris-Bis PAGE result

What should I do

CCR5 and US28 (cytomegalovirus receptor) receptors

It contains Cys35-Asp343

synostoses (multiple) syndrome 1

IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines

***The amount of Quick T4 DNA Ligase used can be added 1-5 μL as needed

The reaction was carried out at 30 oC for 1 hour